Bacterial Transformation

Extract

[...] Bacterial Transformation Introduction The process of genetic transformation is an important tool used in biotechnology. It has been used in agriculture to create plants that are resistant to different kinds of conditions. In medicine it is being used to try and create bacteria that produce insulin which can be used to treat diabetes. Transformation can also e used to give bacteria the traits to digest oil, which then can be used to clean up oil spills. In nature transformation also accurse by itself, when suddenly bacteria become resistant to antibiotics, when they are introduced to it over and over. [...]

[...] Then we used a sterile loop to pick up a colony of bacteria from starter plate and place it in the +DNA tube and another colony into the tube using a new sterile loop. After we added 10ul of plasmid DNA into the +DNA tube only. Once the plasmid solution has been added we incubated the tubes for 10 minuets in ice. While the tubes were being incubated we went and labeled 4 agar plates as figure 1 illustrates. Figure 1.( Miyazaki, 212) When the 10 minuets were up we heat shocked the tubes in an arm water bath at 42 Celsius for 50 seconds and then quickly placed the tubes back into ice when the 50 seconds were up. [...]