Construction of artificial micro RNA with mismatch PCR to direct silencing of lipase gene
Case study - 7 pages - Biology
Lipase is an enzyme that hydrolyzes rice bran oil into free fatty acids, causing spoilage and restricting its usage as food ingredient. Micro RNA (miRNA) is a post-transcriptional regulator endogenously produced in plants that can be potentially altered to specifically target lipase gene,...
Characterization of Egg White Proteins
Case study - 28 pages - Biology
Egg white or albumin is the biggest fraction of whole egg and contains 88% water, 11% proteins, 0.2% fats and 0.2% minerals. The major constituent of egg white proteins are 60% of ovalbumin, 12% of conalbumin, 11% of ovomucoid, and 3.5% of lysozyme. It also contains small amount of ovoinhibitor,...
Agrobacterium-mediated transformation of rice callus with artificial miRNA construct against lipase to produce transgenic plant with lowered lipase activity
Case study - 9 pages - Biology
Lipase is a lipolytic enzyme causes spoilage of rice bran. Its removal by conventional heating and chemical approaches damages bran quality. This experiment aimed to transform Nipponbare rice callus with ami RNA against lipase using Agrobacterium tumefaciens, regenerate whole transgenic rice...
Functional analysis of NQPT1 and NPQT2 promoters, and analysis using GUS activity the transcriptional control and strength of NQPT2 versus CaMV35S promoters
Case study - 20 pages - Biology
Quinolinate phosphoribosyltransferase (QPT) links the pyridine nucleotide cycle and aspartate pathway by maintaining the availability of nicotinic acid important for de novo synthesis of NAD and alkaloid. QPT is encoded by QPT gene. Gene duplication leads to two different forms of QPT gene which...
The impact of sleep duration on vulnerability to false memory implantation
Case study - 5 pages - Biology
Many studies have been examining effects of sleep loss on developing false memory. Yet, its role in ‘lost-in-the-mall' memory implantation has not been discerned. This study will investigate impact of sleep durations on vulnerability to false memory implantation, in university students....
Exploiting the use of fluorophore in extraction and direct detection of rice's lipase activity
Case study - 5 pages - Biology
Lipase is the major protein in rice that catalyzes endogenous hydrolysis of lipids. Study on lipase activity is crucial in rice bran utilization, as it causes rancidity, severely affects the product quality and tarnishes its economic value. This experiment aimed to isolate lipase from different...
Conventional offset
Case study - 5 pages - Biology
It is the main printing process since the second half of the 20th century, ensuring good quality for medium and large print runs and just about any type of paper and some types of plastic (especially polystyrene). Planográfico process, originating in lithography, he makes an indirect impression:...
Contributions of the dual transmission
Case study - 6 pages - Biology
Redemption is an important factor to grip the viewer to moral values upheld by the film. And redemption of the character is linked to the satisfaction of the viewer. Because we believe the films according to our religious beliefs related to our culture thus, we can see that the end of movie...
E case study: Alzheimer's disease
Case study - 6 pages - Biology
Alzheimer's disease is a dementia disorder in the world in the elderly. Today the disease has spread over 860000 cases in France according to WHO (World Organization of Health) and that number keeps growing each year. Often referred to the twenty-first century, this disease attacks the brain...
The creation of the Vincennes Zoo
Case study - 7 pages - Biology
In 1860, the director of the National Museum of Natural History, Geoffroy Saint-Hilaire, wants to create a schedule to the Menagerie du Jardin des Plantes to study the behavior of animals in a larger and more similar space in their environment original life. The project is mainly driven by a...
Aging case study
Case study - 8 pages - Biology
Aging, also known as senescence, is a physiological process that causes a slow deterioration of the functions of the body. It is characterized general degradation capacity of the body: Psychomotor, immune or reproductive. He 'spares no area of the human body, whether skin, bones, muscles but...
The growth and development of plants
Case study - 2 pages - Biology
In Indonesia there are countries plant species diversity. Circumstances such as climate and soil strongly supports the survival of various plant species. Therefore Indonesian citizen is obliged to be grateful for God's grace. Growth is the increase in the number of cells in an organism....
Homeostatic Mechanisms and Malfunctions Leading to Disorders
Case study - 4 pages - Biology
The internal bodily environment is made up of physiological variables such as body temperature, blood sugar levels and mineral concentrations yet organisms are required to function effectively in this wide range of conditions. Homeostasis is the ability of systems or organism to be able to...
Plastic-Assisted Suicide: How Humans are Killing Themselves
Case study - 2 pages - Biology
You are on vacation and you're walking along a white coastline on a hot August day. You're trying to walk as straight as possible, pretending you're an acrobat on a tightrope high above the circus floor. One heel in front of the other foot's big toe, one by one, slowly combing through grains of...
Functional investigation of QPT promoters using a small gene family from Nicotiana, as the test system
Case study - 30 pages - Biology
The QPT gene codes for the QPTase (enzyme), which is found in both prokaryotes and eukaryotes. The enzyme provides the only route for QA metabolism and is essential in the de novo biosynthesis of NAD. It catalyzes the reaction between QA and 5-phosphoribosyl 1-pyrophosphate (PRPP) in the presence...
Isolation and identification of egg white proteins using biochemical techniques
Case study - 15 pages - Biology
Human beings need a balanced diet of carbohydrates, proteins, fats and numerous other nutrients. Proteins are needed as part of a healthy balanced diet in order to sustain growth, provide structural support, boost the defences of the body and are involved in many vital body functions. Among the...
Separation of Haemoglobin from Bovine Serum Albumin using the SDS-PAGE and Ion exchange chromatography techniques
Case study - 15 pages - Biology
Proteins are large molecules with polar and ionizing groups on their surface. Usually the pH affects the polar charges on the molecule, which causes the proteins to move to its oppositely charged electrode or remain stationary, depending on the buffer chosen. Many different electrophoretic...
Use of selective and differential media to grow and identify bacteria
Case study - 4 pages - Biology
Isolating bacteria in the form of pure cultures is very important in order to obtain individual well distanced colonies which would assist in carrying out further identification and microbiological tests on the bacteria of interest. Producing these bacterial pure cultures can be controlled by...
Malaysian soil-soil texture, pH and salinity determination
Case study - 5 pages - Biology
It is essential for farmers to ensure that the soil in which they grow their crops plants can produce a good yield. Determining the influence of factors such as soil texture, pH and salinity is vital to ensure such a high yield. Soils are physically a mixture of inorganic particles of...
Effects of packaging and storage conditions on the freshness of leafy vegetables (Brassica rapa)
Case study - 6 pages - Biology
A wide range of fresh horticultural products contribute to a major component of the agricultural food produce available for consumption nowadays. But these products are highly perishable and if correct post-harvest measures are not employed, it could lead to drastic reduction in product quality,...
Effect of sucrose holding solution on flower longevity
Case study - 3 pages - Biology
Cut flower plants contribute to a very prosperous industry in many parts of the world. The longevity period of these cut flowers is a very important factor which influences the duration of the shelf-life of the flowers. Different cut flowers may have different longevity periods, depending on the...
Categorization of seed proteins based on polypeptide size and protein solubility, to help enrich plant-seed based diets
Case study - 3 pages - Biology
Seeds such as those of cereals and legumes have always been widely harvested to fuel the ever-growing demand of food and have also become a vital provider of the essential amino acids that cannot be produced by the human body, but are needed for growth and development. Classification of the...
Crop science project report: Seed Germination
Case study - 7 pages - Biology
Cultivation of many crops required for food production, depends on the germination of seeds, although there are exceptions where vegetative propagation is carried out. The seeds of legumes and cereals are themselves some of our most important food sources, but their importance lies within the...
Lab Safety audit of A University Mechatronics Laboratory
Case study - 20 pages - Biology
Workplace safety inspection is important in maintaining safe working environment for students and staff in a laboratory. All students and staff are exposed to lots of possible incidents in the laboratory when rules and regulations are not followed. A workplace safety inspection or audit of...
Risks and hazards involved with the usage of neutron generators
Case study - 3 pages - Biology
Radioactivity has always been a health hazard at work places and research laboratories. This became evident since its discovery by Marie Curie and others, whose very deaths were caused by exposure to radiation. This article analyses the precautions necessary when assembling and analyzing neutron...
Cancer
Case study - 2 pages - Biology
Human body is made up of numerous cell tissues. In the process of damaging these cell tissues, can case various diseases in the body resulting to poor health of the victims. Therefore, it is important for human being to take the recommended nutrition that helps in the building and maintenance of...
Personal review: Rare copy number variants contribute to congenital left-sided heart disease' by Hitz M et-al
Case study - 2 pages - Biology
This appraisal significantly evaluates and assesses the article ‘Rare Copy Number Variants Contribute to Congenital Left-Sided Heart Disease' by Hitz M et-al. This evaluation will primarily abridge the article. Subsequently, this evaluation will momentarily examine the efficiency of the...
The comparison of thermo-stable Alpha-amylase products cloned in Bacillus stearothermophilus and Bacillus subtilis.
Case study - 4 pages - Biology
The enzyme Alpha-amylase accounts for about 30% of total enzyme production in the world and contributes to numerous industrial applications, extending from general products such as detergents, paper, beer and textiles to clinical biology, health science and even the pharmaceutical industry...
Bio-Fuel Enzyme Lab Report
Case study - 10 pages - Biology
When talking about enzymes, also known as proteins, you are talking about 3-dimensional structures in your body that act as catalysts, which speed up chemical reactions. If enzymes did not exist in our body these chemical reactions would be occurring at a slow enough rate to where our body would...
Advanced technologies for rapid, large scale and cost-effective sequencing of genomes
Case study - 3 pages - Biology
While low-cost, revolutionizing computers and gadgets seem to dazzle our eyes every time we look around, a recent and fairly rapid progression in cost-effective genome analyses is drawing quite a lot of attention, the world over. Similar to electronics, the prices for rapid sequencing of genomes...
