Studying the soybean nodulation at McGill University

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Studying the soybean nodulation at McGill University

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Abstract

This summer I had the great opportunity to do my three months training in McGill University in Quebec, Canada. It is one of the oldest Canadian universities, considered one of the most prestigious universities in Canada and one of the best in North America. According to the Times Higher Education Supplement it is the best Canadian school and it is among the 25 top universities of the world. With Concordia academy, McGill is the one of the two English universities of Montreal. McGill is divided into two campuses. The main campus is set upon 32 hectares at the foot of Mount Royal in Montreal's downtown district. A second campus—Macdonald Campus—is situated on 650 hectares of fields and forested land in Sainte-Anne-de-Bellevue, 32 km west of the downtown campus. McGill has 21 faculties and professional schools and offers degrees and diplomas in over 300 fields of study. The university also has field research stations in Mont-Saint-Hilaire and Schefferville, Quebec; Axel Heiberg Island in Nunavut; and Holetown, Barbados.

Presentation of the oldest Canadian university: McGill University.
1. McGill university history.
2. Academic profile.
3. McGill hierarchical organization.
4. Strategies and financial aspects.
The Soybean nodulation.
1. The nodulation and the plant hormone defense response.
2. Materials and methods.
3. Results and analysis.
Conclusion.
References.

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[...] At that point, its sole purpose was to administer the McGill bequests on behalf of the college. The Royal Institution for the Advancement of Learning continues to exist and is the "legal person" that runs the university and its various constituent bodies, including the former Macdonald College (now Macdonald Campus), Royal Victoria College (the former women's college turned residence) and the Montreal Neurological Institute. The Institution's name appears on all cheques cut by the university Early years The first classes were held in Burnside Place, James McGill's country home, until the 1840s when the university began construction on its first buildings, the central and east wings of the Arts Building. [...]

[...] The seeds were planted on sterilized vermiculite (Figure 11) in two trays (one for nodulating soybean and another for non-nodulating), whose bottom was covered with plastic. All was kept moist during all the germination. Trays were put in growth chamber (temperature: photoperiod: 16h, Humidity not controlled) during more or less 5 days and watered each day with about one litter of distilled water. For the first two experiments, I used old seeds which gave bad plants with stunted, wrinkled and brown first two leaves. [...]

[...] During my training period at McGill University, I took part in the project of a master student, Keldeagh Lindsay. His work was articulated around two main questions: 1. What is the effect on salicylic acid levels if lipo-chito- oligosaccharide (LCO) is sprayed on the leaves of soybean (Glycine max)? 2. Is there a difference between nodulating and non-nodulating soybean with respect to this effect? We used two kinds of soybean, one was able to do nodulation and the other was a mutant unable to do it. [...]

[...] www.forumsci.co.il/HPLC/progrm.html Consulted the 24/10/06 McGill university website. [on line]. www.mcgill.ca. Consulted on 09/06 Appendices Appendix Hoagland's solution formula In a clean 20 l jug put: distilled water 5 l 0.5 M K2SO4 8.71 g 1 M MgSO4 4.81 g 0.05 M Ca(H2PO4) 2.34 g 0.01 M CaSO4 5.44 g Mix with a magnetic stirrer until all the chemicals are dissolved Complete the volume with distilled water (15 Mix with a magnetic stirrer to homogenize Wash with antibacterial soap some 5 l jugs Put in the 5 l jug: precedent solution 1.5 l distilled water 1.5 l Fe EDTA 1.5 ml *Micronutrients 1.5 ml NH4NO3 7.5 ml Shake before using and reserve at *Micronutrients: H3BO3 MnCl2 ZnSO4 CuSO4 H2MgO4 Appendix Harvest data # Experiment : 1 Date : August 2nd Time : 0h Label : 1Nod+/ Control # sample t1 Weight Methanol volume Label : Nod+/LCO # sample t1 Weight Methanol volume Label : 2Nod -/Control # sample t1 Weight Methanol volume Label : Nod -/LCO # sample t1 Weight Methanol volume 1 Nod +(Nodulating soybean Nod - (Non-nodulating soybean # Experiment : 1 Date : August 4th Time : 48h Label : Nod+/Control Weight Methanol Weight Methanol volume volume Label : Nod+/LCO Weight Methanol Weight Methanol volume volume Label : Nod-/Control Weight Methanol Weight Methanol volume volume Label : Nod-/LCO Weight Methanol Weight Methanol volume volume # Experiment : 2 Date : August 22nd Time : 0h Label : Nod+/Control # sample t1 Weight Methanol volume Label : Nod+/LCO # sample t1 Weight Methanol volume Label : Nod-/Control # sample t1 Weight Methanol volume Label : Nod-/LCO # sample t1 Weight Methanol volume # Experiment : 2 Date : August 23rd Time : 24h Label : Nod+/Control Weight Methanol Weight Methanol volume volume Label : Nod+/LCO Weight Methanol Weight Methanol volume volume Label : Nod-/Control Weight Methanol Weight Methanol volume volume Label : Nod-/LCO Weight Methanol Weight Methanol volume volume # Experiment : 2 Date : August 24th Time : 48h Label : Nod+/Control Weight Methanol Weight Methanol volume volume Label : Nod+/LCO Weight Methanol Weight Methanol volume volume Label : Nod-/Control Weight Methanol Weight Methanol volume volume Label : Nod-/LCO Weight Methanol Weight Methanol volume volume # Experiment : 3 Date : August 30th Time : 0h Label : Nod+/Control # sample t1 Weight Methanol volume Label : Nod+/LCO # sample t1 Weight Methanol volume Label : Nod-/Control # sample t1 Weight Methanol volume Label : Nod-/LCO # sample t1 Weight Methanol volume # Experiment : 3 Date : August 31st Time : 24h Label : Nod+/Control Weight Methanol Weight Methanol volume volume Label : Nod+/LCO Weight Methanol Weight Methanol volume volume Label : Nod-/Control Weight Methanol Weight Methanol volume volume abel : Nod-/LCO Weight Methanol Weight Methanol volume volume # Experiment : 3 Date : September 1st Time : 48h Label : Nod+/Control Weight Methanol Weight Methanol volume volume Label : Nod+/LCO Weight Methanol Weight Methanol volume volume Label : Nod-/Control Weight Methanol Weight Methanol volume volume Label : Nod-/LCO Weight Methanol Weight Methanol volume volume Appendix Salicylic acid level measured by HPLC Salicylic acid levels presented in these tables correspond to the average of the 4 samples harvested in each case. [...]

[...] In order to compare more precisely the difference between nodulating and non-nodulating soybean we can observe the results date by date Comparison between SA concentration in nodulating and non- nodulating soybean At 0h (Figure as I noticed before the SA level is significantly higher for the nod+ than the nod-. For both the SA level did not change a lot at this date. Thus, we can suppose that the LCO do not have an effect immediately which is confirmed by the next results. [...]

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