Temperature Affects E. Coli Growth Rate Positively Over Time

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Temperature Affects E. Coli Growth Rate Positively Over Time

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Temperature, E. Coli, Growth Rate, Positively Over Time

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Abstract

Bacteria are prokaryotic organisms where the DNA is held in a nucleoid, which is a non membrane-enclosed organelle. Many people think bacteria are strictly harmful to us, but in fact they coexist to aid in many daily tasks such as digest food or keep our gums healthy. Although we cannot see a bacteria cell with the naked eye, it can reproduce rapidly through binary fission. Binary fission is a method of asexual reproduction by the cell dividing in half. After a certain point, an accumulation of bacteria cells becomes visible to humans without a microscope.

Much research has been done on the effect of different temperatures on the growth rate with bacteria long before this experiment. There was a study conducted about Microbial Oxidation of CH4 at different temperatures in landfill cover soils. Gunnar Börjesson and his team observed that different methanotroph populations in the soil could produce different concentrations of O2 and CH4, temperature, pH, and nitrogen sources. Methanotrophs are bacteria that use CH4 to gain energy and carbon.

Introduction
E. Coli Growth Rate
Temperature Affects E. Coli Growth Rate Positively Over Time
Materials And Methods
Discussion

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[...] Much research has been done on the effect of different temperatures on the growth rate with bacteria long before this experiment. There was a study conducted about Microbial Oxidation of CH4 at different temperatures in landfill cover soils. Gunnar Börjesson and his team observed that different methanotroph populations in the soil could produce different concentrations of O2 and CH4, temperature, pH, and nitrogen sources. Methanotrophs are bacteria that use CH4 to gain energy and carbon. There are two types of these methanotrophs, called Type I and Type II. [...]

[...] Temperature Affects E. Coli Growth Rate Positively Over Time Authors: Megan Crandell, Brittanny Heilmann, Mack Neal, Kristen Beno Abstract: Escherichia Coli that is cultured in the proper environment will reproduce asexually. To see exactly how temperature affects this growth, we prepared, grew, and observed a culture of a strain of E. Coli in C and C. Measuring the absorption of light in the sample of Escherichia Coli will give one an idea of how much bacteria are present. After a 24-hour period of measuring the absorbance, we found that the Escherichia Coli strain stored at a warmer temperature grew faster than the lower temperature. [...]

[...] This is a very rapid increase that could happen in the digestive tract of a human being due to the temperature of their body. Escherichia Coli is more likely to infect children rather than adults. It brings on symptoms of nausea, severe stomach cramps, and diarrhea. Some people don't develop symptoms until the bacteria have spread rapidly. Escherichia Coli can be obtained through person-to-person contact, through handling raw meet, and through bodies of water. If symptoms do not develop early enough, someone could potentially die. [...]

[...] We predict that higher temperatures are ideal for Escherichia Coli to grow in because the rates of reactions are sped up. The Escherichia Coli grown in higher temperatures will reproduce faster than the Escherichia Coli that is grown in lower temperatures in the same amount of given time. Materials And Methods: Safety precautions. Benches were bleached before and after use, any possible open wounds were covered with Band-Aids, as well as gloves, no food was allowed in the lab, there was no touching of the face, especially the eyes, and all pipette tips were placed in a bio hazard bag. [...]

[...] The absorbance measures the amount of light that is able to pass through, giving an idea of how much bacteria is in the sample. The absorbance rates of the warmer temperature water baths increased much more rapidly than those of the colder temperature water baths. This data suggests that our hypothesis is correct. The growth rate of Escherichia Coli in a warmer temperature is much higher than the growth rate in a cooler temperature. References Bailey, David Lab 9-Bacteria, 1:1-5. [...]

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