Gibberellins (GA) are plant hormones involved with overall plant growth. Brassica Rapa, a flowering plant species, which possesses the wild type phenotype or the rosette, dwarfed, phenotype, was used to explore the effects of Gibberellins on the two varieties' overall height, which can have an application in commercial farming for obtaining larger yields. The GA receptors are physiologically active and the levels of GA are causing the dwarfed appearance in the rosette variety. After seven days of plant growth, varying amounts of hormone were applied to the plants, ranging from 0.0ppm or 0.0 mg/ml GA to 1400ppm or 0.7mg/ml GA. After eighteen days of plant growth, the plants were measured for height and biomass. Data was inconclusive in determining if the GA had any effect on height, which does not necessarily mean GA has no effect, rather something potentially went askew in the experiment. The experiment should be modified and retested in order to find data that is more conclusive.
[...] We hope to see that both phenotypes of plants will show increased growth for height and biomass based on the concentration of GA received, also with adequate amounts of the GA, the rosette seeds will grow to a wild type height. Methods and Materials The lab procedure and materials used are similar to that detailed in Planting and care of Brassica rapa for Plant Hormone Experiment by Olsen et al. (2009); however, we used four watering systems with a total of ninety- six plants (forty-eight of each wild type and rosette variety Brassica rapa). [...]
[...] The average height of the wild type control and the average height of the 0.5 mg/ml rosette showed no significance other than the fact that with GA the rosette was able to obtain a height similar to that of the control wild type. More testing needs to be done to statistically reinforce this notion however. If we were to perform this experiment again, several key components could be modified to improve the experiment. We could try performing this experiment with different plants. [...]
[...] Each level of concentration will be applied to eight wild type and eight rosette type plants. The plants should be labeled with the amount of concentration they received to decrease confusion. After eighteen days height and wet biomass was taken. The ANOVA test was used to analyze the data for significant data. Results A two-way ANOVA test shows that the application of varying amounts of GA resulted in data that was insignificant > 0.05 ) for the average wild- type plant and dwarf plant final heights. [...]
[...] Plants that contain a mutation relating to GA will produce plants that are not the normal height in comparison to the wild-type variety, because the transcription factors were not able to begin transcription. Yang et al. (1996) worked with a pea plant variety with a wild type and a GA1-deficient mutant, which was designated le, to determine what can lead to a dwarf phenotype in the plant species. In the le mutant, the levels of GA1 are greatly reduced, causing very short stemmed pea plants. However, when GA1 is introduced to the plant, the pea plants attain stem length similar to the wild type. Yang et al. [...]
[...] The average wet biomass for both the wild type and dwarf variety Brassica rapa at different concentrations of GA in grams. Discussion From the data, nothing was found to be considered significant. This does not necessarily mean that the experiment was in any way a failure, but it means that the hypothesis was not supported by the data found. General trends can be seen that do support the predictions made, but since it is not significant, the information is not reliable. [...]
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